Increased detection of Mycoplasma pneumoniae infection in children in England and Wales, October 2011 to January 2012. doi: 10.1093/jac/dks457Ĭhalker V, Stocki T, Litt D, et al. Macrolide-resistant Mycoplasma pneumoniae its role in respiratory infection. Pathogenesis of Mycoplasma pneumoniae: An update. 1CIP: Capital Institute of Pediatrics 2ERY: Erythromycin tested alone 3RSP: Reserpine and agent tested in combination with 20 μg/mL reserpine 4CCCP: Carbonyl cyanide m-chlorophenyl-hydrazone and agent tested in combination with 7.5 μg/mL CCCP 5(+RSP+CCCP): Agent tested in combination with 20 μg/mL reserpine and 7.5 μg/mL CCCP 6AZM: Azithromycin tested alone 7MED: medemycin tested alone.Ĭhaudhry R, Ghosh A, Chandolia A. pneumoniae in addition to the common point mutations in 23S rRNA gene. Conclusion Our study suggests that macrolide efflux pump may contribute to macrolide resistance in M. In assays of the minimal inhibitory concentrations (MIC) of macrolide antibiotics in the presence of the efflux pump inhibitors caused a significant decrease of MICs, even under detectable levels in some strains. Strikingly, 4 of 30 SNPs causing non-synonymous mutations were clustered in macrolide-specific efflux system gene macB encoding macrolide-specific efflux pump protein of the ATP-binding cassette transporter family. Results A total of 56 single nucleotide polymorphisms (SNPs) were identified in 10 clinical isolates in comparison to the reference strains M129 and FH. The role of the macrolide-specific efflux transporter was assessed by efflux-pump inhibition assays with reserpine and carbonyl cyanide m-chlorophenyl-hydrazone (CCCP). pneumoniae with macrolide resistance were sequenced by Illumina HiSeq2000 platform. Methods The whole genomes of 10 clinical isolates of M. The aim of this study was to investigate other possible mutations involved in macrolide resistance in M. However, these mutations alone do not fully explain the high resistance rates in Asia. Abstract: Objective Mutations in 23S rRNA gene are known to be associated with macrolide resistance in Mycoplasma pneumoniae ( M.
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